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TDO2 (NM_005651) Human Recombinant Protein
TDO2 (NM_005651) Human Recombinant Protein from Leading Biology
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TDO2 (NM_005651) Human Recombinant Protein
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Description
Recombinant protein of human tryptophan 2, 3-dioxygenase (TDO2)
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Product Specs
Item
TDO2 (NM_005651) Human Recombinant Protein
Company
Leading Biology
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Catalog Number
PH40688M5
Format
Liquid
Conjugate/Tag
C-Myc/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Quantity
20 ug
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
NCBI Full Gene Name
tryptophan 2,3-dioxygenase
NCBI Gene Aliases
HYPTRP, TPH2, TDO, TRPO, TO
Research Area
Metabolic pathways, Tryptophan metabolism
Molecule Name
TDO2
Type
Recombinant Proteins
Source/Expression System
HEK293T
Molecular Weight
47.7 kDa
Species
Human
Activity
The specific activity of TDO2 was determined by monitoring Kynurenine formation from the N-formylkynurenine based on the absorbance at 492nm. The N-formylkynurenine was produced from a conversion of tryptophan with TDO2. The reactions were carried out at 37°C for 40min in 100ul of the reaction volume containing 200mM PBS, pH7.5, 1mM ascorbic acid, and 1.25mM L-tryptophan as the substrate with various amounts of TDO2. The reaction was terminated by adding 50ul of 30% (w/v) trichloroacetic acid. The sample was further incubated for 30min at 60°C and centrifuged at 12000 rpm for 15 min. The supernatant was used to mix with an equal volume of Ehrlich's reagent (2% p-dimethylaminobenza-ldehyde in glacial acetic acid) to measure the absorbance at 492 nm after 10min incubation
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Leading Biology
2600 Hilltop DR
Building G, B Suite C138
Richmond
,
CA
94806
United States
Phone
: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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Website:
leadingbiology.com
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TDO2 (NM_005651) Human Recombinant Protein from Leading Biology
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